Forensic toxicological research is concerned with the collection and storage of blood samples. In the experimental technique, the samples of four people have collected for 4 hrs. Before consumption of alcohol and sequential analysis was done for 6 hrs. After consumption at regular intervals. After subsequent analysis blood was transferred and collected in different sets. Whole blood was collected and Disodium ethylenediaminetetraacetate was added as an anticoagulant. Plasma samples containing EDTA and sodium fluoride samples after centrifugation were stored. Sample analysis was done after 24 h and 8 days at room temperature and after refrigeration. Gas chromatography was used to determine the concentration of alcohol before and after consuming it. The alcohol levels are found to be different in plasma and whole blood. Whole blood showed less concentration of ethanol than plasma. This indicates that there is no effect on the concentration of ethanol by adopting various preservation techniques.