Document Type : Research Article

Authors

Department of Physical Chemistry, School of Chemical Sciences, University of Madras, Guindy Campus, Guindy, Chennai- 600025, Tamil Nadu, India

Abstract

Capecitabine (CPT) is an oral antineoplastic prodrug of 5-Flurorouracil (5-FU), administered for the treatment of metastatic breast and colorectal cancers. Detection of trace quantity of Capecitabine in pharmaceutical drug dosages is very crucial and essential owing to its life threating toxic adverse effects. In this study, an effective conducting nanohybrid namely, MWCNT-PAMAM (G3)-AuNps was developed and characterized by Raman, FT-IR, SEM and HR-TEM techniques. The developed conducting nanohybrid was used for fabrication of effective and stable active electrode viz., GCE-MWCNT-PAMAM (G3)-AuNps which in turn demonstrated for effective sensing of trace quantity of Capecitabine i.e., at a concentration of 5 x 10-12 M under lower potentials. The reduction of Capecitabine was investigated through cyclic voltammetry in the presence of H2SO4 (pH 1.54) as supporting electrolyte. The presence of Capecitabine exhibited an irreversible reductive peak potential at ~0.835 V which was observed from mixed diffusion-adsorption controlled processes. The mechanism for electrochemical-chemical reduction, trace and rapid determination of Capecitabine are reported. This newly developed electrode has a potential to sense/ detect the Capecitabine at the concentration of 5 x 10-12 M under lower potentials. Further, it is expected that there is a strong scope for quality control in pharmaceutical formulates. Copyright © 2017 VBRI Press.

Keywords

1.Heidelberger, C.; Chaudhuari, N. K.; Daneberg, P.; Mooreh,
D.; Griesbach, L.; Duschinsky, K.; Schnitzer, R. J.; Pleven,

E.
Nature. 1957, 179, 663.
2.
O'Shaughnessy, J. A.; Kaufmann, M.; Siedentopf, F.;
Dalivoust, P.; Debled,
M.; Robert, N. J.; Nadia Harbeck, N.
Oncologist
. 2012, 17, 476.
3.McKendrick
, K.; Coutsouvelis, J. Expert Opin
Pharmacother. 2005,6, 1231.

4.
Dyar, S.; Moreno-Aspitia, A. Breast Cancer(Auckl).2011; 5,
239.

5.
Schüller,J.;Cassidy, J.; Dumont,E.;Roos,B.;Durston, S.;
Banken
,L.;Utoh,M.;Mori,K.;Weidekamm, E.; Reigner,B.
Cancer Chemother. Pharmacol.,
2000, 45, 291.
6.Miwa, M.; Ura, M.; Nishida, M.; Sawada, N.;Ishikawa, T.;
Mori, K.; Shimma, N.; Umeda, I.; Ishitsuka, H. Eur J Cancer
1998, 34, 1274.

7.Couch, L. S. B.; Groteluschen, D. l.; Stewart, J.A.; Mulkerin,
D. L. Clin Colorectal Cancer. 2003, 3, 121.

8.
Fontanella, C.; Aita, M.; Cinausero, M.; Aprile, G.; Baldin,
M. G.; Dusi, V.; Lestuzzi, C.; Fasola G.; Puglisi, F.
Onco
Targets Ther.
2014, 7, 1783.
9.Sideris, S.; Loizidou, A.; Georgala, A.; Lebrun, F.; Gil, T.;
Awada. P.; Piccart, P.; Cardoso, F. Acta Clin Belg.2013,
68,135.

10.Yokokawa, T.; Kawakami, K.; Mae, Y.; Sugita, K.;
Watanabe, H.; Suzuki, K.; Suenaga, M.; Mizunuma, N.;
Yamaguchi, T.; Hama, T. Ann Pharmacother.2015, 49,
1120.
11.Nikolic-Tomasevic, Z.; Jelic, S.; Cassidy, J.; Filipovic-
Ljeskovic, I
.;Tomasevic, Z. Cancer Chemother Pharmacol.
2005
, 6, 594.
12.Piórkowska, E.; Kaza, M.; Fitatiuk, J.; Szlaska, I.; Pawinski,
T.; Rudzki, P J. Pharmazie, 2014, 69, 500.

13.Deenen, M. J.; Rosing, H.; Hillebrand, M. J.; Schellens, J. H.;
Beijnen, J. H. J Chromatogr B Analyt Technol Biomed Life
Sci.2013, 30, 913.

14.Montange, D.; Bérard, M.; Demarchi, M.; Muret, P.;
Piédoux, S.; Kantelip, J. P.; Royer, B. J Mass Spectrom.
2010, 45, 670.

15.Vainchtein, L. D.; Rosing, H.; Schellens, J. H.; Beijnen, J. H.
Biomed Chromatogr.2010, 24, 374.

16.Deng, P.; Ji, C.; Dai, X.; Zhong, D.; Ding, L.; Chen, X. J
Chromatogr B Analyt Technol Biomed Life Sci.2015, 989,
71.

17.Wang, X.; Zheng, K.; Feng, X.; Xu, C.; Song, W. Sens
Actuators B Chem.2015, 219, 361.

18.Pattar, V. P.; Nandibewoor, S. T. J Taibah Univ Sci. 2016,
10, 92.

19.
Badawy, W. A.; El-Ries, M. A.; Mahdi, I. M. Talanta. 2010,
82
, 106.
20.Gupta, V. K.; Jain, R.; Agarwal, S.; Mishra, R.; Dwivedi, A.
Anal Bioche. 2011, 410, 266.

21.Gowda, J. I.; Nandibewoor, S. T.Asian J Pharm Sci. 2014, 9,
42.

22.
Kurbanoglu, S.; Dogan-Topal, B.; Uslu, B.; Can, A.; Ozkan,
S. A.
Electroanalysis. 2013, 25, 1473.
23.Iijima, S. Nature. 1991, 354, 56.

24.
Zhao, Q.; Gan, Z.; Zhuang, Q. Electroanalysis. 2004,14,
1609.

25.Cao, Q.; Rogers, J. A. Nano Res.2008, 1, 259.

26.Zhang, W.; Zhang, Z.; Zhang, Y. Nanoscale Res Lett.2011,
6, 555.

27.
Liu, Z.; Yang, K.; Lee, S. T. J.Mater.Chem.2011, 21, 586.
28.
Murugan, E.; Vimala, G. J. Phys. Chem. C.2011, 115,
19897.

29.Thakur, V. K.; Thakur, M. K. Chemical Functionalization of
Carbon Nanomaterials: Chemistry and Applications; CRC
Press, 2015, pp 592-593.

30.Caminade, A. M.; Majoral, J. P. Chem Soc Rev. 2010, 39,
2034.

31.Tomalia, D.A.; Baker, H.; Dewald, J.; Hall, M.; Kallos, G.;
Martin, S.; Roeck, J.; Ryder. J.; Smith, P. Polym J.1985, 17,
117.

32.
Sutriyo; Mutalib, A.; Ristaniah; Anwar, E.; Radji, M.;
Pujiyanto, A.; Purnamasari, P.; Joshita, D.; Adang, H. G.

Macromol Symp.
2015, 353, 96.
33.Zhang, Y,; Liu, X.; Li, L., Guo,Z.; Xue, Z.; Lu, Z. Anal.
Methods.2016, 8, 2218.

34.Niu
, Y.; He, J.;Li,Y.; Zhao,Y.; Xia,C.; Yuan,G.;
Zhang,L.;
Zhang,Y.; Yu, C. Microchim Acta. 2016, 183,
2337

35.Kalanur, S. S.; Seetharamappa, J.; Mamatha, G. P.; Hadagali,
M. D.; Kandagal, P.B. Int. J. Electrochem.Sci., 2008, 3, 756.

36.
Shanmugharaj, A. M.; Bae, J. H.; Lee, K . Y.; Noh, W. H.;
Lee S. H.; Ryu, S. H.
Compos. Sci. Technol.2007, 67,1813.
37.Pan, B.; Cui, D.; Xu, P.; Ozkan, C.; Feng, G.; Ozkan, M.;
Huang, T.; Chu, B.; Li, Q.; He, R.;Hu, G.
Nanotechnology
2009,
20, 9.
38.Filho, A. G. S.; Jorio, A.;Samsonidze, G. G.; Dresselhaus,
G.; Saito, R.;Dresselhaus, M. S. Nanotechnology. 2003, 14,
1130.

39.Neelgund, G. M.; Oki, A.; Luo, Z.Colloids Surf B
Biointerfaces. 2012, 100, 215

40.Buang, N. A.; Fadil, F.; Majid, Z. A.; Shahir, S. Dig J
Nanomater Biostruct. 2012, 7, 33.

41.Mohammed, M. R.; Haider, A. J.; Ahmed, D. S.
Eng.
&Tech.J.
2014, 32, 427.
42.
Murugan, E.; Vimala, G. J Colloid Interface Sci.2011, 357,
354.

43.Neelgund, G. M.; Oki, A. Appl Catal B.2011, 110, 99.

44.Zhang, W.; Bas, A. D.; Ghali, E.; Choi, Y. Trans. Nonferrous
Met. Soc. China.
2015, 25, 2037.
45.Ghoneim, M. M.; Abdel-Azzem, M. K.; El-Desoky, H. S.;
Ghoneim, A. M.; Khattab, A. E. J. Braz. Chem.Soc.2014,
25, 1407.

46.Bard, A.J.; Faulker, L.R. Electrochemical Methods:
Fundamentals and Applications; John Wiley: New York,
2002.

47.
Batchelor-McAuley, C.; Goncalves, L. M.; Xiong, L.; Barros,
A. A.; Richard G. Compton, R. G.
Chem.Commun.2010, 46,
9037.

48.
Gosser, D. K. Cyclic voltammetry: simulation and analysis of
reaction mechanisms; Wiley: USA, 1993.

49.Laviron, E.; Roullier, L.; Degrand, C.; J. Electroanal. Chem.
1980, 112, 11.

50.Gupta, R.; Guin, S. K.; Aggarwal, S. K. RSC Adv.2012, 2,
1810.

51.Shamsipur, M.; Najafi, M.; Hosseini, M. R. M.
Bioelectrochemistry.2010, 77, 120.