Biocompatible silver (Ag) nanoparticles (NPs) were demonstrated using silver nitrate (AgNO3) and egg white extracted (EWE). Egg white mediated AgNPs were characterized and study its antimicrobial activity against human pathogenic bacteria (HPB), and under UV-light irradiation, photocatalytic degradation of methylene blue (MB) has been studied. Results showed that EWE-AgNPs act as remarkable antimicrobial agent against several Gram- Positive and Negative HPB strains. Maximal growth inhibition zone in Salmonella typhimurium (NCIM 2501) and a minimal inhibition zone in Bacillus subtilis (NCIM 2920) were observed at concentration of 80μg mL-1. High activity of photocatalytic degradation of methylene blue (MB) at about 94.67% was observed under UV-light irradiation when the reaction time was extended 30 min. The multi-functionality of EWE-AgNPs may be useful for many industrial applications such as catalysis to waste water treatment in near future. Copyright © VBRI Press.